- Case report
- Open Access
- Open Peer Review
Giant osteoclasts in patients under bisphosphonates
© Mac-Way et al.; licensee BioMed Central Ltd. 2014
- Received: 4 February 2014
- Accepted: 23 June 2014
- Published: 8 July 2014
Bisphosphonates have been widely used for treatment of high bone resorption states. It lowers bone turnover by inhibiting osteoclasts bone resorption with various mechanisms of actions: inhibition of osteoclast formation and attachment to the bone surface, induction of metabolic injury, alteration of vesicle trafficking and induction of osteoclast apoptosis. Bone biopsies studies from patients under bisphosphonates have shown that some resorption parameters are decreased as expected but the number of osteoclasts seems not to be necessarily decreased. The description of osteoclasts morphology from patients treated with bisphosphonates has rarely been reported in the literature.
We describe in this paper two patients treated with bisphosphonates from whom iliac crest bone biopsies have shown large, multinucleated and apoptotic osteoclasts that were not associated with bone resorption activities. The characteristics of these osteoclasts are described and the literature reviewed.
The appropriate recognition of these giant osteoclasts in bone tissues from patients treated with bisphosphonates is of primary importance for bone pathologists and should not be interpreted as signs of increased bone resorption as seen in hyperparathyroidism, bone cancer or Paget’s disease of bone.
- Giant osteoclast
- Bone biopsy
- Bone resorption
Bisphosphonates (Bps) constitute a major class of drugs used to treat various metabolic bone diseases including osteoporosis, metastatic or post-transplant bone disease. Since their introduction more than thirty years ago, many studies have shown that they mainly act through inhibition of osteoclasts (OC) bone resorption: inhibition of OC formation  or attachment to the bone surface , induction of metabolic injury to OC , alteration of vesicle trafficking and/or induction of OC apoptosis . The anti-osteoclastic effects of nitrogen-containing Bps (including alendronate (ALN) and risedronate (RIS)) result from inhibition of the enzyme farnesyl pyrophosphate synthase that ultimately leads to OC death . The decrease in circulating markers of bone resorption in Bps-treated patients has been extensively documented, using measurements of collagen breakdown products in urine or serum  and has been used to monitor patients compliance or predict Bps efficacy . While bone histomorphometry studies mentioned the reduction of resorption rate , period  or lacunae depth  in patients treated with Bps, the number of OC has been reported to be lowered, normal or even increased. In fact, the description of their morphology has rarely been reported in the literature. In this paper, we report two cases in which bone biopsies from patients under Bps showed unusual giant, multinucleated OC that were not associated with resorption activity. These OC are characterized and current literature is reviewed.
Evolution of biological parameters and BMD in patient 1
Deoxypyridinoline (8.2-20.2 nmol/mmol creat)
Intact PTH (10-65 pg/mL)
25-(OH) D (20-90 nmol/L)
Alkaline phosphatase (30-125 U/L)
Histomorphometric parameters for patient 1 and 2
21.9 ± 3.3
2.0 ± 1.2
0.72 ± 0.12
7.0 ± 4.1
24.5 ± 7.5
0.0435 ± 0.060
3.80 ± 1.70
Oc S/BS (%)
1.4 ± 0.5
Evolution of biological parameters and BMD in patient 2
CTX (250–4500 pmol/L)
Intact PTH (10–65 pg/mL)
25-(OH) D (20–90 nmol/L)
Phosphatase (11.6-30.6 U/L)
In this report, we described large multinucleated OC on the edge of bone trabeculae observed on bone biopsies from two patients under Bps therapy. These patients were treated with ALN and RIS for respectively 38 and 26 months. These OC had an average length of 92 μm, mostly detached from bone surface and were not associated with resorption lacunae. No mineralization defects were seen in both patients (normal osteoid volume and mineralization lag time) and OC number was either normal or increased. We observed 2 or 3 of these giant OC per slide that are not usually accounted for OcN/BA determination. Furthermore, these large OC had an apoptotic appearance with a clear nuclear fragmentation and nuclear peripheral beading when stained with DAPI (4′-6-Diamidino-2-phenylindole, data not shown). The BFR was decreased as expected in both patients as well as the biochemical markers of bone resorption while under Bps. The osteoid bone, bone marrow cavity and collagen structure were all well characterized for appropriate diagnosis to rule out other bone pathologies such as hyperparathyroidism, Paget’s disease of bone or neoplasia.
The first paper describing similar OC morphology was published in the New England Journal of Medicine in which the authors reported these giant OC in patients treated with ALN . They reported the results of a 3-year prevention trial of ALN in postmenopausal women . Fifty-one bone biopsies were performed and they described that 27% of OC seen in these biopsies were giant, multinucleated and detached from bone surface with pyknotic nuclei. They found an increased OC number that correlated with the cumulative ALN dose and concluded that long-term ALN treatment was associated with increased OC number but some of them were undergoing apoptosis.
To better describe the phenotype of these OC, a review was published in which the authors explain the characteristics of these giant OC seen from patients under Bps . They reported the presence of these giant OC from iliac crest bone specimen of a 55 year-old woman treated with an aminobisphosphonate for 4 years and they discuss the differential diagnosis including Paget’s disease of bone, hyperparathyroidism, giant cell tumor and fibrous dysplasia. They specify the unique and common characteristics of these OC morphology: often more than 40 nuclear profiles compared to 2–8 nuclear profiles found in normal OC, detachment from the bone surface and up to 30% apoptotic.
Recently, Jobke et al have reported the same description as ours in osteoporotic patients treated with nitrogen-containing Bps during 3 years . They also described a normal number of OC in these patients that is consistent with our histomorphometry results. These giant OC were exactly similar to our description and the one provided previously.
By our description and those made by others, it seems therefore plausible that the presence of these non-resorbing giant OC are directly related to the mechanism of action of the Bps. It has already been shown that nitrogen-containing Bps interfere with farnesyl pyrophosphate synthase in the mevalonate pathway resulting in a reduced geranylgeranyl diphosphate that is required for the prenylation of Guanosine triphosphate binding proteins, hence preventing formation of the ruffled border of the OC [15, 16]. This leads to cytoskeletal malfunction and ultimately death by apoptosis. The decrease in OC resorption capability under Bps therapy could delay OC apoptosis allowing the fusion of OC precursors and produce these large multinucleated and apoptotic OC as proposed by Weinstein et al . In this view, it is therefore not surprising that OC number was reported to be normal or even increased in our patients. Indeed, this was already noted in animal studies where RIS and ALN inhibited bone resorption at doses tenfold lower than what were required to reduce OC number . In ALN treated rats, multinucleated OC that exhibited poorly organized ruffled borders and detached from the bone surface have also been reported . It is important to note that these giant OC are seen not only in patients treated with nitrogen-containing Bps but also with amino-Bps as in our case. The identification of giant multinucleated OC in bone specimens from patients treated with Bps is of primary importance as it should not be interpreted as markers of increased bone resorption. Indeed, the absence of resorption capability, the detachment from bone surface and the presence of apoptosis markers characterise these OC and their presence should not be identified as active OC. Although our patients were different from the cases described in the literature since they had secondary cause of osteoporosis, our findings are nevertheless identical to previously reported cases.
Bisphosphonates decrease bone resorption by inducing OC apoptosis and often allow appearance of multinucleated and giant OC that can be observed on patients bone tissue. The recent description of these OC gives us a better understanding on their mechanisms of action and is essential to recognise for all bone pathologists. Indeed, these giant OC should not be interpreted as markers of increased bone remodelling, which could lead to a false diagnosis of Paget’s disease of bone, hyperparathyroidism bone disease or even bone tumour.
Written informed consent was obtained from the two patients for publication of this Case report and any accompanying images. A copy of the written consent is available for review by the Editor of this journal.
Dr Fabrice Mac-Way is a recipient of the “Vascular Calcification and Mineral Metabolism in Chronic Kidney Disease” award from the Kidney Foundation of Canada.
- Hughes DE, MacDonald BR, Russell RG, Gowen M: Inhibition of osteoclast-like cell formation by bisphosphonates in long-term cultures of human bone marrow. J Clin Invest. 1989, 83: 1930-1935.View ArticlePubMedPubMed CentralGoogle Scholar
- Lowik CW, van der Pluijm G, van der Wee-Pals LJ, van Treslong-De Groot HB, Bijvoet OL: Migration and phenotypic transformation of osteoclast precursors into mature osteoclasts: the effect of a bisphosphonate. J Bone Miner Res. 1988, 3: 185-192.View ArticlePubMedGoogle Scholar
- Fleisch H, Russell RG, Francis MD: Diphosphonates inhibit hydroxyapatite dissolution in vitro and bone resorption in tissue culture and in vivo. Science. 1969, 165: 1262-1264.View ArticlePubMedGoogle Scholar
- Coxon FP, Benford HL, Russell RG, Rogers MJ: Protein synthesis is required for caspase activation and induction of apoptosis by bisphosphonate drugs. Mol Pharmacol. 1998, 54: 631-638.PubMedGoogle Scholar
- Russell RG: Bisphosphonates: from bench to bedside. Ann N Y Acad Sci. 2006, 1068: 367-401.View ArticlePubMedGoogle Scholar
- Garnero P, Shih WJ, Gineyts E, Karpf DB, Delmas PD: Comparison of new biochemical markers of bone turnover in late postmenopausal osteoporotic women in response to alendronate treatment. J Clin Endocrinol Metab. 1994, 79: 1693-1700.PubMedGoogle Scholar
- Eastell R, Barton I, Hannon RA, Chines A, Garnero P, Delmas PD: Relationship of early changes in bone resorption to the reduction in fracture risk with risedronate. J Bone Miner Res. 2003, 18: 1051-1056.View ArticlePubMedGoogle Scholar
- Eriksen EF, Melsen F, Sod E, Barton I, Chines A: Effects of long-term risedronate on bone quality and bone turnover in women with postmenopausal osteoporosis. Bone. 2002, 31: 620-625.View ArticlePubMedGoogle Scholar
- Recker RR, Delmas PD, Halse J, Reid IR, Boonen S, Garcia-Hernandez PA, Supronik J, Lewiecki EM, Ochoa L, Miller P, Hu H, Mesenbrink P, Hartl F, Gasser J, Eriksen EF: Effects of intravenous zoledronic acid once yearly on bone remodeling and bone structure. J Bone Miner Res. 2008, 23: 6-16.View ArticlePubMedGoogle Scholar
- Chavassieux PM, Arlot ME, Reda C, Wei L, Yates AJ, Meunier PJ: Histomorphometric assessment of the long-term effects of alendronate on bone quality and remodeling in patients with osteoporosis. J Clin Invest. 1997, 100: 1475-1480.View ArticlePubMedPubMed CentralGoogle Scholar
- Weinstein RS, Roberson PK, Manolagas SC: Giant osteoclast formation and long-term oral bisphosphonate therapy. N Engl J Med. 2009, 360: 53-62.View ArticlePubMedPubMed CentralGoogle Scholar
- McClung M, Clemmesen B, Daifotis A, Gilchrist NL, Eisman J, Weinstein RS, Fuleihan G-H, Reda C, Yates AJ, Ravn P: Alendronate prevents postmenopausal bone loss in women without osteoporosis. A double-blind, randomized, controlled trial. Alendronate Osteoporosis Prevention Study Group. Ann Intern Med. 1998, 128: 253-261.View ArticlePubMedGoogle Scholar
- Jain N, Weinstein RS: Giant osteoclasts after long-term bisphosphonate therapy: diagnostic challenges. Nat Rev Rheumatol. 2009, 5: 341-346.View ArticlePubMedPubMed CentralGoogle Scholar
- Jobke B, Milovanovic P, Amling M, Busse B: Bisphosphonate-osteoclasts: changes in osteoclast morphology and function induced by antiresorptive nitrogen-containing bisphosphonate treatment in osteoporosis patients. Bone. 2014, 59: 37-43.View ArticlePubMedGoogle Scholar
- Rogers MJ, Gordon S, Benford HL, Coxon FP, Luckman SP, Monkkonen J, Frith JC: Cellular and molecular mechanisms of action of bisphosphonates. Cancer. 2000, 88: 2961-2978.View ArticlePubMedGoogle Scholar
- Halasy-Nagy JM, Rodan GA, Reszka AA: Inhibition of bone resorption by alendronate and risedronate does not require osteoclast apoptosis. Bone. 2001, 29: 553-559.View ArticlePubMedGoogle Scholar
- Alakangas A, Selander K, Mulari M, Halleen J, Lehenkari P, Monkkonen J, Salo J, Vaananen K: Alendronate disturbs vesicular trafficking in osteoclasts. Calcif Tissue Int. 2002, 70: 40-47.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6890/14/31/prepub
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