Mapping mitochondrial heteroplasmy in a Leydig tumor by laser capture micro-dissection and cycling temperature capillary electrophoresis

Table 2 Primers used to amplify fragments suitable for detecting mutation by CTCE

			Template,fragment#			Annealing
#	Start(bp)	End(bp)	fromfirstPCR	“Forward” primer(5′–3′)	“Reverse” primer(5′–3′)	Temperature (°C)
1	16569	25	1	^aTGCATGGAGAGCTCCCGTGAGTGG	CCCCTTAAATAAGACATCACGAT	52
2	42	126	1	^aATTAACCACTCACGGGAGCTCTC	AGGATGAGGCAGGAATCAAAGAC	55
4	131	181	1	^aCACCCTATGTCGCAGTATCTGTC	CACACTTTAGTAAGTATGTTCGC	55
6	483	153	1	^aGGGGTTAGCAGCGGTGTGTGTGTG	TCCCACTCCCATACTACTAATCT	55
7	530	633	1	^aTACCCAGCACACACACACCGCTG	CAAACCTATTTGTTTATGGGGTGA	55
8	673	705	1	^aTTAGAGGGTGAACTCACTGGAAC	GGTTTGGTCCTAGCCTTTCTATT	58
82	7031	1734	10	^aACGACACGTACTACGTTGTAGCC	AATATGATAGTGAAATGGATTTT	52
84	7340	7416	10	^aCTTTCTTCCCACAACACTTTCTC	TCTCAAATCATGAAAATTATTAAT	55
125	11029	11086	16	^aTTAGGAGGGGGGTTGTTAGGGGGT	CATCCCTCTACTATTTTTTAACC	58
127	11193	11243	16	^aACCAGCCAGAACGCCTGAACGCA	GGTGTTGTGAGTGTAAATTAGTG	55
128	11283	11311	16	^aTGTGCCTGCGTTCAGGCGTTCTGG	TAATCATATTTTATATCTTCTTC	60
130	11437	11492	16	^aTTGACCCAGCGATGGGGGCTTCGA	GAGCCAACAACTTAATATGACTA	55
176	15201	15257	22	^aAGAATCGTGTGAGGGTGGGACTGT	AGTAATTACAAACTTACTATCCG	60
177	15274	15377	22	^aAGTAGACAGTCCCACCCTCACAC	GGTGATTTTATCGGAATGGGAGG	60
178	15394	15448	22	^aCTAGGAATCACCTCCCATTCCGA	TAATGTCATTAAGGAGAGAAGGAA	55
181	15761	15864	22	^aACCTCCTCATTCTAACCTGAATC	CAGGCCCATTTGAGTATTTTGTTT	55
184	16080	16130	23	^aCAAGTATTGACTCACCCATCAAC	ACAGGTGGTCAAGTATTTATGGTA	57
185	16170	16272	23	^aGTGGGTGAGGGGTGGCTTTGGAGT	CCAATCCACATCAAAACCCCCTC	56
187	16263	16366	23	^aAACTGCAACTCCAAAGCCACCCC	CCCTATCTGAGGGGGGTCATCCAT	58

ISSN: 1472-6890