Fig. 2

H&E (a), D2-40 (b) and CD-34 (c) staining of consecutive tissue section. This sample was scored as lymphovascular invasion negative by H&E. However, CD34 staining (c) clearly showed the presence of tumor cells within CD34 stained vessel and was scored as blood vessel invasion positive. Lymphatic invasion was scored as negative (b). Noted that IHC was able to distinguih between lymphatic and blood vessel invasion. (200× magnification)