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Table 1 Summary of the seven off the shelf nucleic extraction kits evaluated in this study, including the key differences between each method

From: Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: a trade off between quantity and quality?

Extraction kit Manufacturer Extracted Material Input tissue amount Elution Volume Purification Method Level of automation Proteinase K digestion DNase I digestion Geometric mean Yield (ng)
RNeasy FFPE Qiagen RNA Up to 40 μm 30 μl Silica Column Manual 15 min at 56 °C,
15 min at 80 °C
15 min at room temperature in the cell lysate 398.0
Arcturus Paradise plus RNA extraction and isolation Life technologies RNA Up to 40 μm 12 μl Silica Column Manual 5 h at 37 °C 20 min at room temperature on the column 197.0
Maxwell 16 LEV RNA FFPE Kit Promega RNA Up to 10 μm 50 μl Paramagnetic beads Automated 15 min at 56 °C,
60 min at 80 °C
15 min at room temperature in the cell lysate 231.5
AllPrep DNA/RNA FFPE Qiagen RNA & DNA Up to 40 μm RNA 30 μl DNA 50 μl Silica Column Manual 15 min at 56 °C 15 min at room temperature on the column RNA
267.7
DNA
10.1
Arcturus PicoPure DNA extraction kit Life technologies DNA 1.5–2.0 μg tissue 150 μl Single tube extraction Manual 24 h at 65 °C
(lyophilised before use)
N/A 172.6
QIAmp DNA FFPE tissue Qiagen DNA Up to 80 μm 50 μl Silica Column Manual 60 min at 56 °C,
60 min at 80 °C
N/A 40.9
Maxwell 16 FFPE Tissue LEV DNA Purification Kit Promega DNA Up to 50 μm 50 μl Paramagnetic beads Automated Overnight at 70 °C N/A 88.3