Table 1 Specifications for satisfactory spreading of soft versus calcified tissue samples in adult vascular tissues and fetal samples
Type of tissue | Collagenase II digestion time | Collagenase II working concentration (Ua/ml) | Acetic acid 45 % maceration tIme | Spread sections size (max) |
|---|---|---|---|---|
Adult hard | 24 h | 10U/1 ml | 30 min | 0.1 mm |
Adult soft | 18 h | 10U/1.5 ml | 10 min | 0.3 mm |
Fetal hard | 12 h | 10U/1.5 ml | 20 min | 0.2 mm |
Fetal soft | 1–4 h | 10U/1.5 ml | 10 min | 0.3 mm |