5-type HPV mRNA versus 14-type HPV DNA test: test performance, over-diagnosis and overtreatment in triage of women with minor cervical lesions

Table 9 Test-performance of the 5-type mRNA test and 13–14 types DNA tests in delayed triage and reflex testing of women with minor cytological lesions and CIN3+ as outcome

Ref.	Data collection	Year publ.	Country	Study design	Timing HPV test	Length f-up (mo)	Diagnosis	HPV test	N	N HPV positive	N Met for biopsy	CIN3+
Ref.	Data collection	Year publ.	Country	Study design	Timing HPV test	Length f-up (mo)	Diagnosis	HPV test	N	N HPV positive	N Met for biopsy	Sens.	Spes.	PPV	NPV
6	July 2005– Dec. 2009	2013	Norway	Case-series	Delayed triage	≤36	Repeat ASCUS/LSIL	HC II 5 mRNA	2150 1543	1504 510	1 184 435	NR
7	Jan. 2004– Dec. 2006	2011	Italy	Head-to-head	Reflex testing	≤2	ASCUS/ LSIL	HC II 5 mRNA	795 755	614 204	377 132	NR
10	Jan. 2012– Sept. 2012	2014	Norway^a	Head-to-head	Delayed triage	≤33	Repeat ASCUS/LSIL	COBAS 5 mRNA	281 281	92 37	65 26	100 75.0	77.8 91.6	6.2 11.5	100 99.6
11	Aug. 2005– Jan. 2007	2008	UK	Head-to-head	Reflex testing	Same day	≤ mild dyskaryosis	HC II 5 mRNA	567 558	NR	NRe	100 89.4	26.0 72.8	11.1 23.2	100 NR
12	Sept. 2007– Oct. 2009	2012	UK	Head-to-head	Reflex Testing	Same day	≤ mild dyskaryosis	HC II 5 mRNA	670 641	526 272	NRe	100 80.9	NR	9.2 16.6	100 NR
16	NR	2010	Canada	Head-to-head	Reflex testing	≤6	ASCUS/ LSIL	HC II 5 mRNA	781 781	619 328	NRe	NR
A	Jan. 2012– Sep. 2013		Norway^a	Head-to-head	Delayed testing	≤33	Repeat ASCUS/LSIL	COBAS 5 mRNA	564 564	171 105	105 77	100 100	80.0 85.2	13.9 16.3	100 100

A Present study
Sens Sensitivity; Spes. Specificity; PPV positive predictive value; NPV negative predictive value; NR Not reported; NRe Not relevant. All women had colposcopy regardless of HPV result
^aOnly solved cases are included in test-performance analysis

ISSN: 1472-6890