Extracellular vesicles: potential applications in cancer diagnosis, prognosis, and epidemiology

BMC Clinical Pathology

Table 3 Methods for isolating extracellular vesicles (EVs)

EV Isolation Method	Sample	~ Time required for isolation	Reference/Company
Deferential centrifugation	Serum, urine and cell culture supernatant	2-15 h	[79]
Deferential centrifugation	Cell culture supernatant or saliva	2-15 h	[108,109]
Density gradient centrifugation	Cell culture supernatant	24 h	[80]
Sequential membrane filtration	Cell culture supernatant and biofluids	24 h	[81,110]
Nanomembrane	Urine	<2 h	[82]
Size exclusion chromatography	Plasma	20 min	[111]
Microfluidics	Cell culture supernatant	-	[112]
Nanoshearing	Cell culture supernatant and serum	-	[46]
ExoCap	Cell culture supernatant and biofluids	30 min	MBL International
ExomiR	Cell-free biofluids’ exosomal RNA	20 min	Bioo Scientific
Exo-spin	Cell culture supernatant, urine and saliva	3 h	Cell Guidance Systems
ExoQuick	Serum, plasma, ascites, urine, CSF and cell culture supernatant	2-15 h	System Bioscinces
miRCURY	Serum, plasma, cells, urine and CSF	2 h	Exiqon
Total exosome isolation	Cell culture supernatant and biofluids	2 h	Life Technologies
PureExo	Serum, plasma and cell culture supernatant	2 h	101Bio
ME Kit based on Vn96 peptide binding to heat shock proteins on exosomes	Serum, plasma, urine, cell culture supernatant	30 min	New England Peptide
Streptavidin-biotin-specific antibody to a known antigen on exosome	Cell culture supernatant	>12 h	Life Technologies
Anti-tetraspanin antibody-magnetic bead based	Cell culture supernatant	>12 h	Life Technologies
Anti-EpCAM-antibody magnetic bead based	Cell culture supernatant	>4 h	Life Technologies

ISSN: 1472-6890