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Figure 4 | BMC Clinical Pathology

Figure 4

From: A reliable method for the detection of BRCA1 and BRCA2 mutations in fixed tumour tissue utilising multiplex PCR-based targeted next generation sequencing

Figure 4

GeneRead V.1 reproducibility of analyses. Coverage at 100x depth was reproducible at the recommended DNA input, but as the DNA amount fell below about 1 ng, data were less reproducible. At lower DNA input amounts, BRCA status was less reproducible. ++ positive in all replicates (verified BRCA positive); −− negative in all replicates (negative); +−− positive in first analysis, negative in replicates (artefact - considered negative); +F positive in 1 replicate, but no NGS result in the replicate.

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BMC Clinical Pathology

ISSN: 1472-6890

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