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Table 2 Correlation of RNA degradation time with capillary electrophoresis features and mRNA quantities

From: Improved Methodology for Assessment of mRNA Levels in Blood of Patients with FMR1 Related Disorders

RNA feature Predominant size of
RNA detected and
amplicon length
(for mRNA only)
0 to 18 hours comparison
p values
0 to 24 hours comparison
p values
0 to 96 hours comparison
p values
Combined comparison
p values
18S % of total area (n = 53) 1700 bp 0.001*** 0.001*** 0.001*** 0.001***
28S % of total area (n = 53) 3770 bp 0.001*** 0.001*** 0.001*** 0.001***
5S % of total area (n = 53) 160 bp 0.043** 0.001*** 0.386 0.001***
Fast region % of total area (n = 53) ____ 0.717 0.92 0.001*** 0.001***
Inter-peak region % of total area (n = 53) ____ 0.001*** 0.001*** 0.001*** 0.001***
Post region % of total area (n = 53) ____ 0.36 0.16 0.316 0.42
Lower Marker % of total area (n = 53) ____ 0.256 0.322 0.072* 0.001***
Small fragmentation region % of total area (n = 53) ____ 0.12 0.046** 0.074* 0.026**
28S:18S ratio (n = 53) ____ 0.553 0.082* 0.001*** 0.001***
RQI ____ 0.223 0.024** 0.001*** 0.001***
DNMT1 mRNA (au) (n = 41) 5084 bp – 142 bp 0.173 0.006** 0.004** 0.001***
FMR1ex.3.4 mRNA (au) (n = 43) 4350 bp – 122 bp 0.265 0.001*** 0.001*** 0.003**
FMR1ex13.14 mRNA (au) (n = 43) 4350 bp – 89 bp 0.024** 0.013** 0.012** 0.001***
GUS mRNA (au) (n = 52) 2200 bp – 80 bp 0.169 0.001*** 0.001*** 0.001***
B2M mRNA (au) (n = 35) 976 bp – 114 bpo 0.093* 0.13 0.571 0.01**
GAPDH mRNA (au) (n = 52) 1310 bp – 110 bp 0.117 0.001*** 0.001*** 0.001***
  1. *Generalized estimating equations (GEE) were used. Data from samples with different levels of total RNA degradation (early, moderate, high) are shown as the p-values of the chi square test (p < 0.1*; p < 0.05**; p < 0.001***) and the sample size (n).