Table 2 Summary of specific antisera and immunohistochemistry conditions
From: The possible role of cell cycle regulators in multistep process of HPV-associated cervical carcinoma
Protein | Antibody | Dilution & incubation | Antigen pretreatment | Positive control | Stain |
|---|---|---|---|---|---|
Cyclin D1 | P2D11F11 Novocastra | 1:50 | Microwave | Colon cancer | Nuclear |
Cyclin E | 13A3 Novocastra | 1:80 | Microwave | Breast cancer | Nuclear |
CDK4 | C-22 Santa Cruz Biotechnology | 1:300 | Microwave | Colon cancer | Nuclear |
P53 | DO7 DAKO | 1:50, 2 h | Microwave | Breast cancer | Nuclear |
P21 waf | SX118, DAKO | 1:50, 2 h | Microwave | Epidermis | Nuclear |
Mdm-2 | Monoclonal IF2 Oncogene | 1:20, 2 h | Microwave | sarcoma | Nuclear |
P27 | SX53G8, DAKO | 1:80, 1 h | None | Breast cancer | Nuclear |
P16 | 6H12 Novocastra Lab | 1:40 | Microwave | Epidermis | Nuclear |
Rb | Rb1(recognizes phosphorylated & non-phosphorylated protein) DAKO | 1:80 | Microwave | Retinoblastoma | Nuclear |
Ki-67 | MIB 1, Dako | 1:50 | Microwave | Breast cancer | Nuclear |