Figure 4

Immunohistochemical staining of liver neutrophils. Mice were pretreated with vehicle (i.e. normal saline), DMPP (1 mg/kg), or nicotine (1 mg/kg) prior to the onset of ischemia (90 min), followed by 24 h of reperfusion. Immunostaining of the liver sections was performed using a specific anti-neutrophil antibody. A matched antibody isotype IgG was used as a negative control antibody. Neutrophils are presented by a dark brown stain. Images are representative of three separate experiments with similar results.