Figure 5

Cervical Condyloma Hybridized with HPV Probe. A. A cervical condyloma hybridized with DAKO wide spectrum probe. Diffuse signals (brown arrows) are present in the upper layers and occasional punctate signal is detectable in basal/parabasal layers. AEC was applied for 10 minutes. The authors have previously noted punctate signals in cervical condylomas in mid-/superficial layers [2], and Huang et al. [14] have also reported punctate signals deep in the basal layers of a laryngeal papilloma. B. A section of the same condyloma treated as A . except for use of DAB as substrate (applied for 3 minutes). Diffuse and punctate signals are evident however background staining that includes 'punctate' signals at nuclei circumferences and associated with cytoplasm compromises data interpretation. Scale Bar: 10μm