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Figure 4 | BMC Clinical Pathology

Figure 4

From: Tannic acid label indicates abnormal cell development coinciding with regeneration of renal tubules

Figure 4

Transmission electron microscopy of renal tubules generated in (a,c,e) CO 2 Independent Medium and (b,d,f) Leibovitz’s L-15 Medium for 13 days. Specimens fixed by (a,b) conventional glutaraldehyde show only bright tubule cells. In contrast, specimens fixed in (c-f) glutaraldehyde containing tannic acid reveal a heterogeneously composed epithelium consisting of bright and dark cells. e,f) High magnification shows intact bright and dark cells. The apical plasma membrane faces a lumen (arrow), while the basal side rests on a basal lamina (asterisk). Luminal and lateral plasma membranes are separated by a tight junction (arrow head).

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