Figure 2

Examination of the polymerase chain reaction (PCR) for T-cell receptor gamma from DNA extracted from formalin-fixed, paraffin-embedded whole tissue sections (current lymph node as well as tissue from the initial diagnosis) using a high-resolution fragment length analyser. Monoclonal gene rearrangements are identified as prominent, single-sized amplification products. This is seen in both samples, with a fragment length analysis showing a “peak” (black arrow) at 191 base pairs length in two subsequent runs, suggesting clonally-related relapse. The bottom line (red) reveals the size of the fragment size.