Table 2 Overall concordance analysis between manual and automated HER2 for four continuous HER2 /CEP17 FISH parameters
Manual HER2 FISH | Automated HER2 FISH | Correlation analysis | Linear regression analysis | ||||
|---|---|---|---|---|---|---|---|
Parameter | Mean ± SD | Mean ± SD | Pearson correlation coefficient | P value | Slope | Intercept | R square |
HER2 copy number | 6.41 ± 4.6 | 5.87 ± 4.2 | 0.947 | 0.01 | 0.863 | 0.338 | 0.898 |
CEP17 copy number | 2.02 ± 0.43 | 2.24 ± 0.59 | 0.705 | 0.01 | 0.962 | 0.299 | 0.497 |
HER2/CEP17 ratio | 3.13 ± 2.19 | 2.56 ± 1.71 | 0.943 | 0.01 | 0.738 | 0.255 | 0.890 |
Proportion of carcinoma cells with HER2/CEP17 ratio >2.2 (%) | 46.6 ± 41.6 | 41.1 ± 39.4 | 0.970 | 0.01 | 0.919 | −1.732 | 0.942 |