Table 1 Studied proteins, primary antibodies, source and dilutions of antibodies, staining patterns, and interpretation (cut-off) criteria used in the present study

Cell adhesion molecules

  E-cadherin

HECD-1¹

1:30

20 min / ER1

60 min

IRS ≥5

C, C/M

  P-cadherin

56²

1:200

20 min / ER2

O/N

IRS ≥5

C, C/M

Cell assembly molecules

  Fascin-1

IM20¹

1:400

20 min / ER1

O/N

5%

C

  Cytokeratin Multi

AE1/AE3¹

1:100

10 min / Enzyme 1

20 min

1%

C

Cell cycle regulators

  Cyclin D1

SP4³

1:80

20 min / ER1

30 min

IA (5%)

N

Cell growth molecules

  VEGF-A

VG1⁴

1:75

20 min / ER2

60 min

5%

C

  VEGF-C

18-2255, pl⁵

1:250

20 min / ER1

O/N

5%

C

  Phospho-mTOR^Ser2448

49 F9⁶

1:30

20 min / ER1

20 min

5%

C, C/PN

Cell proliferation molecules

  Ki67

MIB1⁴

1:70

15 min / ER2

20 min

IA (13%)

N

Cell receptors

  EGFR

31 G7⁵

1:50

8 min / Enzyme 2

20 min

10%

M, C/M

  VEGFR-2

55B11⁶

1:450

20 min / ER2

O/N

5%

C, C/N

  VEGFR-3

KLT9¹

1:50

15 min / ER1

O/N

5%

C

DNA repair molecules

  ERCC1

8 F1²

1:300

15 min / ER2

30 min

50%

N, C/N

  p53

DO-7⁴

1:100

20 min / ER1

20 min

50%

N, N/C

Inflammation mediators

  COX-2

4H12¹

1:300

20 min / ER1

O/N

IRS≥3

C

Microtubule assembly

  MAPT (Tau)

2B2.100⁷

1:100

20 min / ER1

O/N

5%

C, N, C/N

Molecules with kinase activity

  Phospho-AKT^Ser473

D9E⁶

1:150

20 min / ER2

O/N

5%

C, N, C/N

  Phospho-GSK-3β^Ser9

5B3⁶

1:50

20 min / ER1

O/N

5%

C, N, C/N

  Phospho-p44/42 MAPK^{Thr202/Tyr204}

20 G11⁶

1:1000

20 min/ ER1

O/N

5%

C, N, C/N

Transcriptional activator or repressor

  p63

4A4¹

1:50

20 min / ER2

60 min

5%

N

Tumor suppressor

  PTEN

6H2.1⁴

1:300

20 min / ER2

60 min

10%

C, N, C/N