Methyl-binding domain protein-based DNA isolation from human blood serum combines DNA analyses and serum-autoantibody testing

Table 1 Overview of sample processing

Parameter	source 1	source 2	source 3
Origin	AIT	Austrian Red Cross	AKH
Sample type	serum/plasma	serum	serum
Test persons	control individuals age and sex matched	control individuals age and sex matched	control/breast cancer
Quantity	serum, n = 12 plasma, n = 8	serum, n = 48	non-canc., n = 30 cancer met., n = 12 cancer mal., n = 30 cancer ben., n = 12
Isolation	MBD/silica*	MBD/silica*	MBD/silica**
Analyses	DNA quantification multiplexed PCR test qPCR measurments array based autoanitbody tests	DNA quantification multiplexed PCR test	DNA quantification

AIT, Austrian Institute of Technology; AKH, Vienna General Hospital; cancer met., serum samples from cancer patients with a metastasizing tumor; cancer mal., patients with an malign tumor; cancer ben., derived from patient with a benign tumor; *DNA of all samples isolated by both isolation methods; ** DNA from 72 samples of this group was isolated using "silica", 12 using "MBD" MBD, methyl-binding domain protein; silica, silica membrane-based isolation

ISSN: 1472-6890