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Figure 1 | BMC Clinical Pathology

Figure 1

From: COLD-PCR enhanced melting curve analysis improves diagnostic accuracy for KRAS mutations in colorectal carcinoma

Figure 1

Melting Curve Assay Design. PCR primers are designed within introns of the functional KRAS (Ki-Ras2) gene on chromosome 12 to avoid amplification of a KRAS (Ki-Ras1) pseudogene on chromosome 6. A Cy5.5-labeled wild type sensor probe sits over the mutation hotspot at codons 12 and 13. When a KRAS mutation is present, the sensor probe melts off at a lower T m .

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BMC Clinical Pathology

ISSN: 1472-6890

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