Fig. 4

Comparison of the Agilent RNA assay and our in house PCR assay for assessing RNA quality: a Agilent RNA assay, graph shows Geometric mean RIN values and associated standard error of mean for RNA samples extracted using four different RNA extraction kits from FFPE blocks <6 months of age or between 2 and 3 years of age. N represents the percentage of samples analysed that were assigned a RIN value. b In-house PCR assay, graph shows Mean Cq value for the amplification of three housekeeping genes: ACTB, RPLP0 and RPL19 for sample duplicates, for RNA samples extracted using four different RNA extraction kits from FFPE blocks either <6 months of age (blue) or between 2 and 3 years of age (pink), horizontal black lines represent the mean Cq for the entire sample population, with pink and blue lines for the two subgroups of FFPE blocks, N represents the percentage of samples analysed assigned a Cq value