Transcriptional profiling of degraded RNA in cryopreserved and fixed tissue samples obtained at autopsy

BMC Clinical Pathology

Table 1 Results for RNA Obtained from Cryopreserved Tissue Specimens

Sample	Type	Asterand Quality Grade	RIN^a	RNA Conc (ng/μL)	# Genes Detected^b	Correlation of Replicates (R²)^a
1	Brain	Passed	7.2	550	425	0.88
2	Brain	Passed	8.1	586	411	0.96
3	Brain	Passed	8.1	673	425	0.93
4	Brain	Passed	7.5	635	391	0.91
5	Brain	Passed	6.6	641	391	0.93
	Passing RNA Ave		7.5	617	409	0.92
6	Brain	Failed	3.1	660	395	0.92
7	Brain	Failed	5.8	275	388	0.86
8	Brain	Failed	2.5	740	421	0.92
9	Brain	Failed	6.2	590	415	0.89
10	Brain	Failed	6.5	554	399	0.91
11	Brain	Failed	5.8	598	461	0.93
	Failing RNA Ave		5.0	569	413	0.91

^aRNA Integrity number as assessed by 2100 Bioanalyzer
^bGenes detected over background by Beadstudio software with a certainty of p = 0.99
^cTechnical replicates of same RNA extraction run in separate array experiments

ISSN: 1472-6890