Protein | Antibody [clone, source] | Antibody dilution | Time/Pretreatment | Incubation Time | Cutoff (%) | Staining pattern |
---|---|---|---|---|---|---|
Cell adhesion molecules | ||||||
E-cadherin | HECD-11 | 1:30 | 20 min / ER1 | 60 min | IRS ≥5 | C, C/M |
P-cadherin | 562 | 1:200 | 20 min / ER2 | O/N | IRS ≥5 | C, C/M |
Cell assembly molecules | ||||||
Fascin-1 | IM201 | 1:400 | 20 min / ER1 | O/N | 5% | C |
Cytokeratin Multi | AE1/AE31 | 1:100 | 10 min / Enzyme 1 | 20 min | 1% | C |
Cell cycle regulators | ||||||
Cyclin D1 | SP43 | 1:80 | 20 min / ER1 | 30 min | IA (5%) | N |
Cell growth molecules | ||||||
VEGF-A | VG14 | 1:75 | 20 min / ER2 | 60 min | 5% | C |
VEGF-C | 18-2255, pl5 | 1:250 | 20 min / ER1 | O/N | 5% | C |
Phospho-mTORSer2448 | 49 F96 | 1:30 | 20 min / ER1 | 20 min | 5% | C, C/PN |
Cell proliferation molecules | ||||||
Ki67 | MIB14 | 1:70 | 15 min / ER2 | 20 min | IA (13%) | N |
Cell receptors | ||||||
EGFR | 31 G75 | 1:50 | 8 min / Enzyme 2 | 20 min | 10% | M, C/M |
VEGFR-2 | 55B116 | 1:450 | 20 min / ER2 | O/N | 5% | C, C/N |
VEGFR-3 | KLT91 | 1:50 | 15 min / ER1 | O/N | 5% | C |
DNA repair molecules | ||||||
ERCC1 | 8 F12 | 1:300 | 15 min / ER2 | 30 min | 50% | N, C/N |
p53 | DO-74 | 1:100 | 20 min / ER1 | 20 min | 50% | N, N/C |
Inflammation mediators | ||||||
COX-2 | 4H121 | 1:300 | 20 min / ER1 | O/N | IRS≥3 | C |
Microtubule assembly | ||||||
MAPT (Tau) | 2B2.1007 | 1:100 | 20 min / ER1 | O/N | 5% | C, N, C/N |
Molecules with kinase activity | ||||||
Phospho-AKTSer473 | D9E6 | 1:150 | 20 min / ER2 | O/N | 5% | C, N, C/N |
Phospho-GSK-3βSer9 | 5B36 | 1:50 | 20 min / ER1 | O/N | 5% | C, N, C/N |
Phospho-p44/42 MAPKThr202/Tyr204 | 20 G116 | 1:1000 | 20 min/ ER1 | O/N | 5% | C, N, C/N |
Transcriptional activator or repressor | ||||||
p63 | 4A41 | 1:50 | 20 min / ER2 | 60 min | 5% | N |
Tumor suppressor | ||||||
PTEN | 6H2.14 | 1:300 | 20 min / ER2 | 60 min | 10% | C, N, C/N |