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Table 1 T-cell rearrangements were identified in the patient’s autopsy specimens by TCR PCR (a) and TCR Southern blotting assay (b)

From: B-chronic lymphocytic leukemia showed triple transformation, to diffuse large B cell, CD20-negative, and T-cell neoplasm during ofatumumab treatment: a case report

(a) T-cell receptor rearrangement PCR assay

 TCR chain

Assay region

Cervical

Paraaortic

Liver

 TCR γ (TRG)

Vγ1–8,10/Jγ

+

+

+

Vγ9,11/Jγ

+

 TCR δ (TRD)

Vδ/Dδ/Jδ

 TCR β (TRB)

Vβ/Jβ1,2

+

+

Vβ/Jβ2

+

Vβ/Dβ1,2

+

(b) T-cell receptor rearrangement Southern blotting assay

 TCR chain

 

Cervical

Paraaortic

Liver

 TCR Cγ

 

+

+

 TCR Cδ1

 

a

a

 TCR Cβ1

 

+

+

  1. aNormal rearrangement banding was strikingly weak. The assay suggests gene deletion or other irregularities