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Table 1 Primers used to selectively amplify mtDNA

From: Mapping mitochondrial heteroplasmy in a Leydig tumor by laser capture micro-dissection and cycling temperature capillary electrophoresis

       Annealing
# Start (b p) End (b p) Length (b p) “Forward” primer (5′–3′) “Reverse” primer (5′–3′) Temperature (°C)
23 15924 201 846 aAACCGGAGACGAAAACCTTTTTC aCTTTAGTAGGTATGTTCGCCTGT 51
1 16521 880 928 aCCATAAAGCCTAAATAGCCCACA aCCAACCCTGGGGTTAGTATAGCT 54
10 6917 7671 754 aTGCTCTGAGCCCTAGGATTCATC aTGAGGGCGTGATCATGAAAGGTG 55.5
16 10852 11566 714 aGCCTAATTATTAGCATCATCCCC aATGCCTCATAGGGATAGTACAAG 51
22 15169 15993 824 aGAGGGGCCACAGTAATTACAAAC aTGGGTGCTAATGGTGGAGTTAAA 51
  1. a = tail sequence (CGCCCGCCGCGCCCCGCG)
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