Table 1 Primers used to selectively amplify mtDNA
Annealing | ||||||
|---|---|---|---|---|---|---|
# | Start (b p) | End (b p) | Length (b p) | “Forward” primer (5′–3′) | “Reverse” primer (5′–3′) | Temperature (°C) |
23 | 15924 | 201 | 846 | aAACCGGAGACGAAAACCTTTTTC | aCTTTAGTAGGTATGTTCGCCTGT | 51 |
1 | 16521 | 880 | 928 | aCCATAAAGCCTAAATAGCCCACA | aCCAACCCTGGGGTTAGTATAGCT | 54 |
10 | 6917 | 7671 | 754 | aTGCTCTGAGCCCTAGGATTCATC | aTGAGGGCGTGATCATGAAAGGTG | 55.5 |
16 | 10852 | 11566 | 714 | aGCCTAATTATTAGCATCATCCCC | aATGCCTCATAGGGATAGTACAAG | 51 |
22 | 15169 | 15993 | 824 | aGAGGGGCCACAGTAATTACAAAC | aTGGGTGCTAATGGTGGAGTTAAA | 51 |