Antibodies | Type | Antigen specifications | Company | Locus-Clone | Dilution | Endogenous peroxidase blocking | Antigen unmasking treatment |
---|---|---|---|---|---|---|---|
Ki-67 | Mouse Monoclonal | Perichromosomal layer protein. Identifies cells in G1-S-G2-M phases. | Dako A/S, Copenhagen, Denmark | 10q26.2 MIB-1 | 1:100 | 20 min | Citrate Buffer, heat mediated (4 cycles 750 W, 5 min each) |
Osteocalcin | Rabbit Polyclonal | Bone specific protein. Synthesized by osteoblasts, accumulates in the bone matrix. | Millipore USA | 1p22 | 1:100 | 30 min | Tryton 0.5 % 4 min |
Desmin | Mouse Monoclonal | Muscle specific intermediate filament type III protein. | Millipore USA | 2q35 D33 | 1:100 | 20 min | Citrate Buffer, heat mediated (4 cycles 750 W, 5 min each) |
Vimentin | Mouse Monoclonal | Intermediate filament type III protein expressed in mesenchymal cells. | Millipore USA | 10p13 V9 | 1:80 | 20 min | Not required |