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Table 3 Technical characteristics of the antibodies used for imunohistochemistry

From: The study of calcified atherosclerotic arteries: an alternative to evaluate the composition of a problematic tissue reveals new insight including metakaryotic cells

Antibodies

Type

Antigen specifications

Company

Locus-Clone

Dilution

Endogenous peroxidase blocking

Antigen unmasking treatment

Ki-67

Mouse Monoclonal

Perichromosomal layer protein. Identifies cells in G1-S-G2-M phases.

Dako A/S, Copenhagen, Denmark

10q26.2 MIB-1

1:100

20 min

Citrate Buffer, heat mediated (4 cycles 750 W, 5 min each)

Osteocalcin

Rabbit Polyclonal

Bone specific protein. Synthesized by osteoblasts, accumulates in the bone matrix.

Millipore USA

1p22

1:100

30 min

Tryton 0.5 % 4 min

Desmin

Mouse Monoclonal

Muscle specific intermediate filament type III protein.

Millipore USA

2q35 D33

1:100

20 min

Citrate Buffer, heat mediated (4 cycles 750 W, 5 min each)

Vimentin

Mouse Monoclonal

Intermediate filament type III protein expressed in mesenchymal cells.

Millipore USA

10p13 V9

1:80

20 min

Not required