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Fig. 1 | BMC Clinical Pathology

Fig. 1

From: The study of calcified atherosclerotic arteries: an alternative to evaluate the composition of a problematic tissue reveals new insight including metakaryotic cells

Fig. 1

Adult calcified carotid artery sections EDTA treated (a-b-c) and collagenase treated (c-d). a Haematoxylin-Eosin (HE) staining of a standard section fixed in carnoy and EDTA treated (scale bar 40 μm). The insert in figure a shows the whole arterial section with a diameter of 4 mm, * asterisks highlights the calcified areas. b osteocalcin immunostain of the same tissue fixed in carnoy (scale bar 20 μm) or (c) fixed in formalin (scale bar 10 μm). d-e Osteocalcin immunostaining of the collagenase treated tissue fixed in carnoy. d positive osteoblast and (e) positive osteoclast-like cells, scale bar 5 μm. Nucleus and cytoplasmatic structure are clearly visible in collagenase spread tissue compared to standard section (a and b)

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BMC Clinical Pathology

ISSN: 1472-6890

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