Liver cancer with concomitant TP53 and CTNNB1 mutations: a case report

BMC Clinical Pathology

Table 1 Morphology, immunohistochemistry and mutational status of individual tumor areas

	Area	A1	A2	A3	B	C1	C2	C3	C4	C5	C6	C7	C8	C9	C10
	Size in mm²	7.8	5.54	31.3	65	73.2	16.5	34.5	82.2	20.2	18.9	17.4	28.5	1.94	17.5
Morphology	Solid	+	+	+		+	+	+		+	+		+
	Glandular (cholangiocellular)				+
	Trabecular							+	+			+		+	+
	Clear cell change		+			+									+
	Fatty change											+
	CK19				>50 %		>50 %
IC	CK7				70 %	SC	50 %	20 %	SC	40 %	SC		SC		50 %
	glutamine synthetase	+	+	+
	β-catenin nuclear	+	+	+
	p53	+	+	+
Mutation	CTNNB1 sanger seq	D32	D32	wt	wt	wt	wt	wt	wt	wt	wt	wt	wt	wt	wt
	CTNNB1 deep seq	D32	D32	D32^a	wt	wt	wt	wt	wt	wt	wt	wt	wt	wt	wt
	TP53 sanger seq	IVS8-1	IVS8-1	wt	wt	wt	wt	wt	wt	wt	wt	wt	wt	wt	wt
	TP53 deep seq	IVS8-1	IVS8-1	IVS8-1^a	wt	wt	wt	wt	wt	wt	wt	wt	wt	wt	wt

sanger seq sanger sequencing, deep seq deep sequencing (NGS), wt wild type, SC single cells positive
^alow frequency (<10 %)

ISSN: 1472-6890