Fig. 1
From: Liver cancer with concomitant TP53 and CTNNB1 mutations: a case report
Combined HCC/CCC in a 72 year old male patient with history of hepatitis C and diabetes revealing morphological, immunohistochemical and genetic intratumor heterogeneity. a Gross morphology and slide overview (H&E staining) with annotations of defined tumor areas. b Microscopic images (40x) of different tumor areas (H&E staining, CK7, glutamine synthetase and β-catenin immunohistochemistry). c p53 and β-catenin nuclear positivity in consecutive slides of tumor area A2. d Nucleotide exchanges in CTNNB1 and TP53 genes (TP53 depicted as reverse sequence). e Mutated allele frequencies of double mutated areas A1-3 with number of gene copies analyzed. Reference sequences: NM 1904.1 (CTNNB1) and NM 001126112.2 (TP53)