Figure 6From: A reliable method for the detection of BRCA1 and BRCA2 mutations in fixed tumour tissue utilising multiplex PCR-based targeted next generation sequencingGene coverage and read depth using the Ion Ampliseq BRCA1/2 panel; effect of 25 and 30 PCR cycles. Depth of coverage was generally better at 25Â cycles for the majority of samples compared with 30Â cycles. Pool 3 products were responsible for the majority of low coverage regions.Back to article page