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Figure 2 | BMC Clinical Pathology

Figure 2

From: A reliable method for the detection of BRCA1 and BRCA2 mutations in fixed tumour tissue utilising multiplex PCR-based targeted next generation sequencing

Figure 2

Percentage coverage at 100x depth compared with DNA input using GeneRead V.1. Gene coverage is shown over the range of DNA inputs. Approximately 97% coverage (maximum by design) is achieved when the recommended DNA input of 20 ng per plex is used. If less than 20 ng of DNA input per plex is used >95% coverage is still obtained for 70% (52/75) of samples even with as little as 1 ng input DNA per plex.

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BMC Clinical Pathology

ISSN: 1472-6890

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