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Figure 1 | BMC Clinical Pathology

Figure 1

From: A reliable method for the detection of BRCA1 and BRCA2 mutations in fixed tumour tissue utilising multiplex PCR-based targeted next generation sequencing

Figure 1

Comparison of DNA concentration measurements. DNA quantification data generated by qPCR using 3 different amplicon sizes (41 bp, 129 bp and 305 bp) for all samples studied, and Nanodrop readings for ovarian samples only. The data indicate the level of degradation of DNA in each sample. Ovarian samples appeared to be less degraded than the breast samples as the 41 bp and 129 bp measurements were more similar compared with the marked differential of nearly an order of magnitude observed for the breast samples. Nanodrop readings did not appear to correlate with the qPCR data, generating significantly higher readings as a measure of total DNA rather than amplifiable DNA (ovarian sample data only). Data have been plotted on a log scale to enable a visual comparison.

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BMC Clinical Pathology

ISSN: 1472-6890

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