Table 1 Primers used for amplification and sequencing of the RBL2 gene.
From: pRb2/p130 protein expression and RBL2 mutation analysis in Burkitt lymphoma from Uganda
| Exon | Primer sequence (5'-3') | Annealing temperature | Product size (bp) | |
|---|---|---|---|---|
| 19-1 | Forward | AGGGTGTGCTCAACAAATAC | 50 | 142 |
| Reverse | CTGTTGGAGAATTCTGATGG | |||
| 19-2 | Forward | TCTCTAAAGGTGTATAGAAGTG | 50 | 161 |
| Reverse | GAAAAGCTCCTAATTTTGTC | |||
| 20-1 | Forward | ATTAGCAGCCTTTGTACTGAC | 55 | 148 |
| Reverse | ACTGTTGGCACCTGTGAGG | |||
| 20-2 | Forward | AGCAGTGCTCCTCCCACA | 50 | 174 |
| Reverse | AAAGTATGAAATAATCCCTG | |||
| 21 | Forward | CTCTCTCCCTATCCATTTG | 50 | 143 |
| Reverse | GGACTGTTGCTGAAGTAATAG | |||
| 22-1 | Forward | CTGAGCTATGTGCATTTGCA | 52 | 148 |
| Reverse | TTTGCAGGTGATTCACTTCC | |||
| 22-2 | Forward | AAAGAGAGGAATTCTTTTGG | 54 | 145 |
| Reverse | AGAGTTTAATACAAGAGACT |
