Skip to main content

Advertisement

Table 1 HPV L1 gene amplification by MY09/MY11 PCR primers followed by positive short or long nested PCR

From: Validation of human papillomavirus genotyping by signature DNA sequence analysis

HPV Type MY09/MY11+ MY09/MY11- Total isolates
  Short Long Short_ Long  
6 8 0 12 N 20
11 1 0 0 N 1
16 40 0 28 N 68
18 16 0 5 N 21
31 11 0 4 N 15
32 3 0 0 N 3
33 4 0 1 N 5
35 6 1 0 N 7
39 4 1 7 N 12
40 2 0 3 N 5
45 4 0 5 N 9
51 2 0 0 N 2
52 9 0 16 N 25
53 2 1 0 N 3
54 5 0 13 N 18
56 4 0 11 N 15
58 6 0 3 N 9
59 4 0 8 N 12
61 2 0 5 N 7
62 2 0 5 N 7
66 0 0 9 N 9
67 0 0 1 N 1
68 0 0 1 N 1
69 0 0 2 N 2
70 1 0 5 N 6
71 0 0 2 N 2
72 4 0 2 N 6
73 4 0 7 N 11
74 0 0 1 N 1
81 3 0 4 N 7
83 2 2 1 N 5
84 2 0 1 N 3
86 0 0 4 N 4
87 0 0 1 N 1
91 0 0 1 N 1
Mixed 7 0 21 N 28
Total 158 5 189   352
  1. MY09/MY11+ = 450 bp amplicon visualized on primary PCR gel.
  2. MY09/MY11- = 450 bp amplicon not visualized on primary PCR gel.
  3. Short = 150 bp or 190–200 bp nested PCR amplicon visualized.
  4. Long = 380–395 bp nested PCR needed due to failure of short PCR.
  5. N = not performed.
  6. Underlined are "High-risk" HPVs targeted by Digene hc2 assay.