Figure 2

Concordance of DNA aneuploidy/tetraploidy on standard FC and ICS in BAC. A: BAC shows a DNA aneuploid histogram on FC as well as on ICS. The top panel shows a DNA diploid (2N) peak and an aneuploid peak on FC. The aneuploid peak on standard FC had a DI value of 1.6. The bottom panel shows that on ICS, no diploid peak was seen but a broad aneuploid peak with DI value of 1.69, and a large scatter of cells and some cells with DNA content > 9N (see marker for DI of 4). B: BAC shows a DNA tetraploid histogram on standard FC and ICS. The top panel shows a DNA diploid (2N) peak and a tetraploid peak on standard FC. Note that the diploid peak has a large CV (21.41%), representing a diploid with an overlapping paradiploid peak. The bottom panel shows, on ICS, a small diploid peak but a large broad tetraploid peak with DI value of 1.87. Also note a large scatter of cells with different DNA contents but no cells with DNA content > 9N (X-axis shows IOD values and the vertical lines mark DI values).