Table 1 The effect of exposure to preservative solution for 20 hours on the viability of renal biopsy tissue assessed by MTT reduction and a histology score following CD 10 antibody staining. See text for abbreviations.
Preservative solution | Temp. of incubation | Duration of incubation | Mean optical density readings | % reduction in formazan formation | Histology score (PAS) | Immuno-staining score (CD 10) |
|---|---|---|---|---|---|---|
1. NCS | 1°C | 20 hours | 0.225 | 31.60% | 12 | 2 |
2. PBS | 1°C | 20 hours | 0.151 | 54.10% | 12 | 2 |
3. MEM | 1°C | 20 hours | 0.197 | 40.10% | 7 | 2 |
4. MEM+NCS+Cells | 1°C | 20 hours | 0.253 | 23.10% | 10 | 2 |
5. Water | 1°C | 20 hours | 0.01 | 97.00% | 4 | 0 |
6. Soltran | 1°C | 20 hours | 0.283 | 14.00% | 8 | 3 |
7. MEM+NCS | 1°C | 20 hours | 0.244 | 25.80% | 9 | 3 |
8. None (Control) | 37°C | 0 minutes | 0.329 | - | 11 | 3 |
9. NCS | 37°C | 20 hours | 0.164 | 50.20% | 5 | 1 |
10. PBS | 37°C | 20 hours | 0.029 | 91.20% | 4 | 1 |
11. MEM | 37°C | 20 hours | 0.127 | 61.40% | 6 | 1 |
12. MEM+NCS | 37°C | 20 hours | 0.174 | 47.10% | 4 | 1 |
13. MEM+NCS+Cells | 37°C | 20 hours | 0.179 | 45.60% | 4 | 1 |
14. Water | 37°C | 20 hours | 0.007 | 97.90% | 0 | 0 |
15. Soltran | 37°C | 20 hours | 0.025 | 92.40% | 3 | 1 |