Skip to main content

Table 1 Comparison of HER-2/neu status assessed by FISH and Real-Time PCR in 39 breast cancer samples deemed 2+ by IHC

From: Real-time PCR complements immunohistochemistry in the determination of HER-2/neu status in breast cancer

Sample number FISH Real-Time PCR
  CEP 17 HER-2 Ratio Status Ratio Status
1 1.83 1.83 1.00 - 1.33 -
2 1.45 1.60 1.10 - 0.48 -
3 1.18 1.23 1.04 - 0.65 -
4 1.35 1.27 0.94 - 1.3 -
5 1.48 1.75 1.18 - 1.5 -
6 1.37 1.37 1.00 - 1.74 -
7 2.40 3.21 1.34 - 0.66 -
8 1.88 1.73 0.92 - 1.35 -
9 6.22 5.85 0.94 - 1.46 -
10 1.77 1.75 0.99 - 1.62 -
11 1.28 1.28 1.00 - 1.49 -
12 1.72 1.68 0.98 - 1.52 -
13 1.53 1.50 0.98 - 1.42 -
14 1.80 1.78 0.99 - 1.43 -
15 3.30 3.22 0.98 - 1.18 -
16 2.47 2.45 0.99 - 1.59 -
17 1.73 1.82 1.05 - 0.41 -
18 1.70 1.73 1.02 - 1.53 -
19 1.83 1.93 1.05 - 0.35 -
20 3.03 3.55 1.17 - 1.58 -
21 1.97 1.55 0.79 - 1.65 -
22 1.68 1.72 1.02 - 1.60 -
23 1.40 1.20 0.86 - 1.39 -
24 1.27 1.80 1.42 - 1.77 -
25 1.20 1.18 0.98 - 1.68 -
26 1.07 1.07 1.00 - 0.44 -
27 1.10 1.08 0.98 - 0.76 -
28 1.98 2.40 1.21 - 1.49 -
29 1.85 2.58 1.39 - 1.96 -
30 2.70 3.22 1.19 - 1.76 -
31 1.95 1.97 1.01 - 1.84 -
32 4.17 6.85 1.64 - 1.67 -
33 1.92 2.00 1.04 - 2.07 +
34 1.77 7.13 4.03 + 1.83 -
35 1.93 4.92 2.55 + 1.51 -
36 1.92 6.77 3.53 + 3.91 +
37 1.77 16.70 9.44 + 11.10 +
38 2.00 5.87 2.94 + 2.09 +
39 2.00 8.15 4.08 + 2.39 +
  1. CEP 17 = mean/cell of the chromosome 17 centromere probe signals HER-2 = mean/cell of the HER-2/neu probe signals
  2. FISH Ratio = the ratio between HER-2 and CEP 17
  3. Real-Time PCR Ratio = the normalized ratio between HER-2 and the reference gene (gastrin)
  4. "+" = positive, "-"=negative. Cases (8%) that were discordant between PCR and FISH assays are highlighted.