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Table 2 PCR protocol sensitivities for the detection of HPV-16 in SiHa/C-33A DNA mixtures. SiHa cell DNA (0.1 ng to 100 ng) was mixed with C-33A cell DNA to give final DNA quantities of 2 μg, 1 μg, 0.5 μg, or 0.1 μg. These preparations were tested with up to six different PCR protocols. HPV-16 detection was most successful using by the TDP3 and TDP4 conditions. Data are for protocols tested at 50 PCR cycles. HPV detected, HPV undetected, ND: not done.

From: Touchdown General Primer (GP5+/GP6+) PCR and optimized sample DNA concentration support the sensitive detection of human papillomavirus

  Total DNA in PCR: 2 μg Total DNA in PCR: 1 μg
SiHa DNA 100 ng 25 ng 1 ng 0.5 ng 0.1 ng 100 ng 25 ng 1 ng 0.5 ng 0.1 ng
GP5+/6+
TDP1
TDP2
TDP3
TDP4 ND ND ND ND ND
MRT ND ND ND ND ND ND ND ND ND ND
  Total DNA in PCR: 0.5 μg Total DNA in PCR: 0.1 μg
SiHa DNA 100 ng 25 ng 1 ng 0.5 ng 0.1 ng 100 ng 25 ng 1 ng 0.5 ng 0.1 ng
GP5+/6+
TDP1
TDP2
TDP3
TDP4
MRT ND ND ND ND ND