Table 2 PCR protocol sensitivities for the detection of HPV-16 in SiHa/C-33A DNA mixtures. SiHa cell DNA (0.1 ng to 100 ng) was mixed with C-33A cell DNA to give final DNA quantities of 2 μg, 1 μg, 0.5 μg, or 0.1 μg. These preparations were tested with up to six different PCR protocols. HPV-16 detection was most successful using by the TDP3 and TDP4 conditions. Data are for protocols tested at 50 PCR cycles. 
HPV detected, 
HPV undetected, ND: not done.
HPV detected, 
HPV undetected, ND: not done.|  | Total DNA in PCR: 2 μg | Total DNA in PCR: 1 μg | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
SiHa DNA | 100 ng | 25 ng | 1 ng | 0.5 ng | 0.1 ng | 100 ng | 25 ng | 1 ng | 0.5 ng | 0.1 ng |
GP5+/6+ |
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TDP1 |
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TDP2 |
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TDP3 |
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TDP4 | ND | ND | ND | ND | ND |
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MRT | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
|  | Total DNA in PCR: 0.5 μg | Total DNA in PCR: 0.1 μg | ||||||||
SiHa DNA | 100 ng | 25 ng | 1 ng | 0.5 ng | 0.1 ng | 100 ng | 25 ng | 1 ng | 0.5 ng | 0.1 ng |
GP5+/6+ |
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TDP1 |
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TDP2 |
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TDP3 |
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TDP4 |
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MRT | ND | ND | ND | ND | ND |
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