Figure 6From: Touchdown General Primer (GP5+/GP6+) PCR and optimized sample DNA concentration support the sensitive detection of human papillomavirus Amplification of HPV from abnormal cervical cell DNA samples by TDP3 and TDP4. Seven samples that tested negative for HPV using standard GP5+/GP6+ cycling conditions were assessed with the TDP3 and TDP4 assays. Both TDP3 and TDP4 supported the detection of HPV in sample 4 when 100 ng DNA was subject to PCR but not when 500 ng DNA was in a reaction. HPV was detected in sample 2 by the TDP3 protocol with 100 ng or 500 ng template DNA and by the TDP4 protocol at 500 ng but not 100 ng of sample. RB: reagent blank.Back to article page