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Figure 2 | BMC Clinical Pathology

Figure 2

From: Touchdown General Primer (GP5+/GP6+) PCR and optimized sample DNA concentration support the sensitive detection of human papillomavirus

Figure 2

Southern blot of TDP3 and MRT GP5+/GP6+ PCR products. SiHa cell DNA, 0, 0.1, 0.5, 1.0, 25, or 100 ng, was diluted into a total of 0.1 μg DNA made up with C-33A DNA. After PCR with the TDP3 and MRT protocols and gel electrophoresis, products were electroblotted onto nylon membrane and hybridized with biotin-labeled HPV-16 oligonucleotide probe detected with streptavidin-alkaline phosphatase and NBT/BCIP. The data confirm the ~150 bp amplicons represent HPV sequences.

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