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Figure 9 | BMC Clinical Pathology

Figure 9

From: Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens

Figure 9

CIN III Lesion Hybridized with HPV Probe. A. A CIN III lesion processed using AEC substrate (applied for 10 minutes). Punctate signals are prominent in the basal layers. Diffuse signal is also present. B. A section from the same lesion processed using DAB (applied for 1 minute). Diffuse and punctate signals are detectable. There is also considerable background staining. Note: A. and B. are both slightly over-digested as indicated by the weak hematoxylin staining and loss of tissue morphology. Tissue over-digestion generally resulted in increased background with DAB but with AEC background remained clean.Scale Bar: 10μm

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BMC Clinical Pathology

ISSN: 1472-6890

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