Figure 7From: Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens CIN I Lesion Hybridized with Biotin-Labeled pUC18. A. A CIN I lesion (Figure 6.) hybridized with biotin-labeled pUC18 resulting in a background-free section. AEC was applied for 10 minutes. B. A CIN I lesion (Figure 6.) hybridized with biotin-labeled pUC18 resulting in punctate-like signals around cells and in the cytoplasm. DAB was applied for 3 minutes. This non-specific staining demonstrates that care is required when interpreting DAB signals, and illustrates the necessity to perform proper control assays when using tyramide reagents. Scale Bar: 20μmBack to article page