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Figure 3 | BMC Clinical Pathology

Figure 3

From: Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens

Figure 3

Normal Cervix Hybridized with HPV Probe. A. Normal cervix hybridized with DAKO wide spectrum probe and developed with AEC substrate (applied for 10 minutes) results in a clean background. B. A section from the same normal cervix treated with the same protocol as A. but using DAB (applied for 3 minutes) as substrate. There is generalized brown staining of epithelial cytoplasm and a tendency for 'punctate-like' signal to 'aggregate' around nuclei circumferences. There are occasional 'punctate-like' signals within nuclei and also associated with cytoplasm. Taken together with the data in Figure 4., it is concluded the staining represents non-specific background. Scale Bar: 10μm

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BMC Clinical Pathology

ISSN: 1472-6890

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