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Figure 10 | BMC Clinical Pathology

Figure 10

From: Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens

Figure 10

Carcinoma Hybridized with HPV Probe. A. HPV hybridization signals in a cervical squamous cell carcinoma demonstrated with AEC (applied for 10 minutes). B. A section from the same carcinoma with hybridization signal demonstrated with DAB (applied for 3 minutes). A given signal is more intense with DAB than with AEC, however overall the information about the carcinoma that is gained with DAB is also obtained with AEC as a similar proportion of cells demonstrate 1 or 2 HPV signals after application of either substrate. Background is relatively clean with DAB on this sample. Scale Bar: 10μm

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BMC Clinical Pathology

ISSN: 1472-6890

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