TY - JOUR AU - Evans, Mark F. AU - Aliesky, Holly A. AU - Cooper, Kumarasen PY - 2003 DA - 2003/06/11 TI - Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens JO - BMC Clinical Pathology SP - 2 VL - 3 IS - 1 AB - Over the past five years in situ hybridization techniques employing tyramide amplification reagents have been developed and promise the potential detection of low/single-copy nucleic acid sequences. However the increased sensitivity that tyramide amplification brings about may also lead to problems of background staining that confound data interpretation. SN - 1472-6890 UR - https://doi.org/10.1186/1472-6890-3-2 DO - 10.1186/1472-6890-3-2 ID - Evans2003 ER -