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Table 2 Effect of repeated freezing cycles on TAC values

From: A new automated method for the determination of the Total Antioxidant Capacity (TAC) of human plasma, based on the crocin bleaching assay

Anti-coagulant Number of freeze/thawing cycles
  1 2 3 4 5
K3-EDTA 1.05 ± 0.02 1.00 ± 0.02 1.05 ± 0.03 1.13 ± 0.03 1.22 ± 0.04
Heparin 1.05 ± 0.03 0.95 ± 0.02 1.00 ± 0.03 1.24 ± 0.04 1.32 ± 0.05
  1. Ten different plasma samples from normal individuals, collected on K3-EDTA or heparin, were assayed after a number of 1–5 freeze/thawing cycles, by the automated TAC assay. Values are presented as the mean ± SEM. The target value of non-freezed plasma, collected in a K3-EDTA tube was given in Table 1