Figure 3

Patient #2. GBM cells are halted at stage 1 of ciliogenesis with rare cells progressing to stage 5. (A) Basal body and abnormal stage 1 cilium with absent paired lateral vesicles. Inset cross section through the transition zone from another cell. (B) Basal body and abnormal stage 1 cilium with vertically outstretched distal appendages and no vesicles. (C) Basal body with clear transition zone and abnormal stage 1 cilium with absent vesicles. (D) Rare occurrence of a primary cilium at stage 5 with abnormal destruction of the cilium-pit with cytoplasmic extrusion, darkened microtubules at proximal end of cilium and electron dense collection of material at the transition zone. EM scale bars = 500 nm.