Table 2 Description of antibodies and dilutions utilized for immunohistochemistry
Antibody | Clone | Source | Dilution |
|---|---|---|---|
CD3 | SP7 | THERMO SC. | 1:150 |
CD4 | 4B12 | NOVOCASTRA | 1:150 |
CD8 | C8/144B | DAKO | 1:200 |
CD20 | L26 | NOVOCASTRA | 1:100 |
CD68 | PG-M1 | DAKO | 1:50 |
CD123 | 7G3 | BD Phamingen | 1:100 |
CD163 | 10D6 | NOVOCASTRA | 1:200 |
GATA-3 | L50-823 | BD Phamingen | 1:150 |
FOXP3 | 221D/D3 | SEROTEC | 1:200 |
T-bet | 4B10 | SANTA CRUZ | 1:100 |
Granzyme | GrB-7 | MONOSAN | 1:100 |
CD117 | T595 | NOVOCASTRA | 1:10 |