Table 1 Details of the immunohistochemistry methodology for four antibodies
Antibody | Company | Clone of antibody | Host animal | Antigen retrieval | Antibody dilution | Incubation temperature | Duration of incubation |
|---|---|---|---|---|---|---|---|
KOC/ IMP3 | DAKO | L523S, 69.1 | Mouse Monoclonal | HIER* (Citrate buffer PH 6) | 1:50 | 25°C | 60 min |
S100P | BD Biosciences | 16 | Mouse monoclonal | Proteinase K (10 minutes) | 1:100 | 25°C | 60 min |
Mesothelin | Novocastra | 5B2 | Mouse monoclonal | HIER (Citrate buffer PH 6) | 1:20 | 25°C | 60 min |
MUC1 | Novocastra | MA695 | Mouse monoclonal | HIER (Citrate buffer PH 6) | 1:200 | 25°C | 60 min |