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Table 1 Details of the immunohistochemistry methodology for four antibodies

From: Expression of KOC, S100P, mesothelin and MUC1 in pancreatico-biliary adenocarcinomas: development and utility of a potential diagnostic immunohistochemistry panel

Antibody Company Clone of antibody Host animal Antigen retrieval Antibody dilution Incubation temperature Duration of incubation
KOC/ IMP3 DAKO L523S, 69.1 Mouse Monoclonal HIER* (Citrate buffer PH 6) 1:50 25°C 60 min
S100P BD Biosciences 16 Mouse monoclonal Proteinase K (10 minutes) 1:100 25°C 60 min
Mesothelin Novocastra 5B2 Mouse monoclonal HIER (Citrate buffer PH 6) 1:20 25°C 60 min
MUC1 Novocastra MA695 Mouse monoclonal HIER (Citrate buffer PH 6) 1:200 25°C 60 min
  1. *HIER= Heat Induced epitope retrieval.