Figure 2
From: Tannic acid label indicates abnormal cell development coinciding with regeneration of renal tubules
Confocal fluorescence microscopy on whole mount species labeled for cytokeratin 19 (Troma III) to analyze spatial distribution of generated tubules. As well in (a) CO2 Independent Medium and (b) Leibovitz’s L-15 Medium development of numerous tubules (T) is observed within the artificial interstitium. c,d) Higher magnification depicts that label is detected on all of analyzed cells. Further generated tubules contain a simple epithelium with prismatic cells bordering a lumen (arrow) and a basal lamina (asterisk).