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Table 3 Estradiol immunoassay cross-reactivity

From: Cross-reactivity of steroid hormone immunoassays: clinical significance and two-dimensional molecular similarity prediction

Compound Plasma/serum concentrations Cross-reactivity in Roche assay Likelihood of clinically significant cross-reactivity
Estradiol (endogenous compound) • 0.01 – 0.04 ng/mL (males) [37] 100% High (assay target)
• 0.013 – 0.50 ng/mL (premenopausal females) [37]
• Up to 4.3 ng/mL (pregnancy) [37]
Estrone • Up to 0.06 ng/mL (males) [24] 0.54% Low
• Up to 0.2 ng/mL (females) [24]
Ethinyl estradiol Up to 0.1 ng/mL while on medication [3841] 0.23% Low
Estriol • Up to 18 ng/mL in pregnancy [42] 0.09% Possible contribution in pregnancy
  • Up to 2.4 ng/mL in non-pregnant females [43]