Cross-reactivity of steroid hormone immunoassays: clinical significance and two-dimensional molecular similarity prediction

Table 2 DHEA sulfate immunoassay cross-reactivity

Compound	Plasma/serum concentrations	Cross-reactivity in Roche assay	Likelihood of clinically significant cross-reactivity
DHEA sulfate	• Up to 6,070 ng/mL (infants) [31]	100%	High (assay target)
	• 5 – 4,070 ng/mL (females > 1 year old) [31]
	• 5–4,920 ng/mL (males > 1 year old) [31]
Pregnenolone sulfate	• 21 – 84 ng/mL (adult controls) [32]	2.1%	Low, possible minor effect around time of parturition.
Pregnenolone sulfate	• Up to 1,580 ng/mL in pregnancy [33]	2.1%	Low, possible minor effect around time of parturition.
17-Hydroxyprogesterone	• 0.1 – 2.0 ng/mL (pediatric controls) [22]	0.08%	Low, possible minor effect in 21-hydroxylase deficiency.
17-Hydroxyprogesterone	• Up to 1,005 ng/mL (21-hydroxylase deficiency) [22]	0.08%	Low, possible minor effect in 21-hydroxylase deficiency.
17-Hydroxypregnenolone	• Up to 4.3 ng/ml in healthy females [34, 35]	0.05%	Low
17-Hydroxypregnenolone	• Up to 4.8 ng/mL in healthy males [34, 35]	0.05%	Low
	• Up to 100 ng/mL in premature infants [36]

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