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Table 2 DHEA sulfate immunoassay cross-reactivity

From: Cross-reactivity of steroid hormone immunoassays: clinical significance and two-dimensional molecular similarity prediction

Compound Plasma/serum concentrations Cross-reactivity in Roche assay Likelihood of clinically significant cross-reactivity
DHEA sulfate • Up to 6,070 ng/mL (infants) [31] 100% High (assay target)
• 5 – 4,070 ng/mL (females > 1 year old) [31]
• 5–4,920 ng/mL (males > 1 year old) [31]
Pregnenolone sulfate • 21 – 84 ng/mL (adult controls) [32] 2.1% Low, possible minor effect around time of parturition.
• Up to 1,580 ng/mL in pregnancy [33]
17-Hydroxyprogesterone • 0.1 – 2.0 ng/mL (pediatric controls) [22] 0.08% Low, possible minor effect in 21-hydroxylase deficiency.
• Up to 1,005 ng/mL (21-hydroxylase deficiency) [22]
17-Hydroxypregnenolone • Up to 4.3 ng/ml in healthy females [34, 35] 0.05% Low
• Up to 4.8 ng/mL in healthy males [34, 35]
  • Up to 100 ng/mL in premature infants [36]